

This DLC adds two new Challenge Maps to Batman: Arkham Asylum, taking place in unique settings from Arkham Island: - Heart of Darkness Challenge Map - Hothouse Prey Challenge Map
Following the Joker's attack on Gotham City Hall, he is apprehended by Batman and transported to Arkham Asylum, where several members of the Joker's gang are temporarily held after a fire at Blackgate Prison. Thinking the Joker intentionally turned himself in, Batman enters the asylum with him. The Joker's scheme unfolds when Harley Quinn takes over the security systems, allowing the Joker to flee the facility with the help of a dishonest guard who abducts Commissioner Gordon. The Joker warns that bombs placed throughout Gotham will detonate if anyone attempts to enter Arkham, compelling Batman to operate alone. Pursuing Quinn to the medical wing to save Gordon, Batman is exposed to Scarecrow's fear toxin and experiences terrifying hallucinations, including visions of Gordon's death and the ghostly figures of his parents speaking to him. After defeating Scarecrow, Batman captures Quinn and rescues Gordon. The Joker then leads Batman to Bane, who has been subjected to experiments by Arkham's doctor, Penelope Young. The Joker releases Bane, and Batman is forced to battle him, during which Quinn manages to escape. Later, Batman retreats to a hidden Batcave installation on the island to replenish his equipment.
| PS3 | September 24, 2009 |
| Mac | September 24, 2009 |